Fig. 12.

Apoptosis obtained with 3LL cells after 24-h incubation (a); and obtained with EA.hy926 endothelial cells after 24-h incubation (b) with 50 μM Fisetin NCs, free Fisetin, P407, and DMSO at the same respective concentration. Colour code: (black) late apoptosis, (dark gray) early apoptosis, (light gray) necrosis, and (white) viable cells. Control group: untreated cell groups were added to the mixture (Annexin V-FITC, PI and binding buffer). The figures were treated with GraphPad Prism version 9 from flow cytometry device. Data are mean of triplicate (value ± SD).