Table 3.

Cryopreservation study of the Fisetin NCs. Mean diameter of Fisetin NCs with mannitol as cryoprotectant agent measured by DLS at 25 °C. Comparison with Fisetin NCs prepared in 0%, 5% and 10% w/v mannitol after storage at −80 °C, then thaw at 5 °C before measurement, n = 3.

Time after freezing (day)	Fisetin nanocrystal sample
	no mannitol		mannitol 5%		mannitol 10%
	Size (nm)	PDI	Size (nm)	PDI	Size (nm)	PDI
0	144.6 ± 5.0	0.16 ± 0.01	153.2 ± 6.2	0.17 ± 0.01	162.4 ± 7.2	0.17 ± 0.02
1	599.5 ± 36.4	0.54 ± 0.1	259.2 ± 8.2	0.18 ± 0.01	278.4 ± 18	0.18 ± 0.03
7	nd (visible aggregation)		248.2 ± 5.6	0.15 ± 0.03	265.7 ± 21.1	0.17 ± 0.02
30	nd		287.1 ± 7.2	0.2 ± 0.01	320.2 ± 27.3	0.18 ± 0.01
60	nd		338.7 ± 12.6	0.21 ± 0.03	359.8 ± 7.7	0.2 ± 0.05
90	nd		345.6 ± 26.8	0.22 ± 0.04	395.1 ± 13.2	0.21 ± 0.02
120	nd		384.9 ± 16.2	0.23 ± 0.02	478.5 ± 9.5	0.27 ± 0.03