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. 2022 Nov 5;66:101632. doi: 10.1016/j.molmet.2022.101632

Figure 2.

Figure 2

Low dose proinflammatory cytokines improved insulin secretion in NOD.SCID ZnT8 HE isolated islets. (A) Representative images of immunofluorescent staining of ZnT8 (green) and insulin (red) from 17wk NOD.SCID ZnT8 male mice. Scale = 100 μm. (B) Intraperitoneal glucose tolerance test (IPGTT) of 14wk male NOD.SCID WT, HE and KO mice after 16 h fasting and 2 g/kg glucose load by IP injection; n = 9 per group. (C) In vivo glucose stimulated insulin secretion measured after 4 h fasting; n = 4 per group. (D–F) Ex vivo glucose stimulated insulin secretion assay normalized to insulin content (n = 8–10 per group). (G) The ratio of secreted proinsulin to insulin in response to 20 mM glucose stimulation. Supernatants were collected 30 min after glucose stimulation. Data are expressed as the mean ± SEM, and analyzed by 2-Way ANOVA ∗ p < 0.05, ∗∗∗p < 0.001.