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. 2022 Nov 5;66:101632. doi: 10.1016/j.molmet.2022.101632

Figure 4.

Figure 4

Increased oxygen consumption rate (OCR) upon low dose proinflammatory cytokine treatment in NOD.SCID HE male isolated islets. (A) Representative oxygen consumption rate (OCR) analysis in isolated islets without cytokine treatment and (B) treated with low dose cytokine for 24 h. From each animal 10 size-matched islets per well were analyzed in Seahorse XFe-96 spheroid plates. Islets were acutely exposed to A: 20 mM glucose, B: 4 μM Oligomycin, C: 4 μM FCCP, D: 4 μM Rotenone. n = 7 WT, n = 6 HE and n = 7 KO. Green circle = WT, blue circle = HE and black = KO. (C–F) Basal respiration, maximal respiration, proton leak and ATP production was calculated by Wave 4.0 software. Data are expressed as the mean ± SEM and analyzed by 2-Way ANOVA ∗∗p < 0.01, ∗∗∗p < 0.001.