PRMT6 loss does not affect lipid accumulation in alcohol-fed mice. (A–E) Wild-type (WT), Prmt6+/- (Het), and Prmt6-/- (KO) mice were fed western diet (WD, control) or WD with alcohol in the drinking water (WDA, alcohol) for 16 weeks. N = 3–8 mice per group. (A) Weight change over time in 12 groups of mice. ns, not significant between genotypes. (B) Alcohol intake in female mice and Prmt6 gene expression in livers of wild-type (WT), Prmt6+/- (Het), and Prmt6-/- (KO) mice fed WD or WDA. (C) Liver to body weight ratios. ∗∗P < .01. (D) Representative images of H&E staining from control and alcohol-fed mice. Bars, 200 μm. (E) Liver triglyceride (TG) levels in mice fed WD or WDA diets. ∗P < .05, ns, not significant. Two-way analysis of variance, males genotype (F2,22 = 1.299, P = .29); diet (F1,22 = 5.850, P = .024); interaction (F2,22 = 1.928, P = .16); females genotype (F2,22 = 3.909, P = .0353); diet (F1,22 = 2.187, P = .1534); interaction (F2,22 = 3.952, P = .034).