Figure 2.

Disruption of microbiota rhythmicity in SCN-specific Bmal1-deficient mice (A) Beta-diversity MDS plot based on generalized UniFrac distances (GUniFrac) of fecal microbiota stratified by genotype. (B–C) Circadian profile of alpha diversity (B) and the relative and absolute abundance of major phyla (C). (D) Heatmap illustrating the relative abundance of 412 zOTUs (mean relative abundance >0.1%; prevalence >10%). Data are ordered based on the zOTUs phase in the controls and normalized based in the peak of each zOTU. (E) Significance and amplitude (based on JTK_CYCLE) of all zOTUs (left) and phase (based on cosine regression) distribution (right) in both genotype. Dashed line represent adj. p-value = 0.05 (JTK_CYCLE). (F) Taxonomic tree of zOTUs losing rhythmicity in Bmal1^SCNfl/- mice based on quantitative analyses. Taxonomic ranks were indicated as phylum (outer dashed ring), families (inner circle) and genera (middle names). Each zOTU is represented by individual branches. (G) Circadian profiles of absolute abundance of example zOTUs losing rhythmicity in Bmal1^SCNfl/- mice. Significant rhythms according to cosine-wave regression analysis (p-value ≤ 0.05) are visualized with a solid line, while data connected by dashed line indicate arrhythmicity. n = 6 mice/time point/genotype. Data are represented as mean ± SEM.