Figure 6.

Simulated shift work-associated microbiota promote weight gain and suppress GI clocks (A) Schematic illustration of cecal microbiota transfer from SSW and LD donors (n = 4–5) intro germ free wild type mice. (B) Normalized body weight gain of recipient mice. (C) bar chart illustrated cecum weight in recipient mice. (D–E) Relative expression at CT1 and CT13 of clock genes (D) and clock controlled gene (E, F) in the GI tract of germ free mice (green), germ free receiving SSW (red) and LD controls (black) microbiota. N = 5–6 mice/time point/light condition. Data are represented as mean ± SEM. Mann Whitney U test was used to assess the different in cecum weight. Two-way ANOVA was used to assess the change in body weight and differences in gene expression. Significance ∗ p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.