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. 2022 Nov 10;66:101634. doi: 10.1016/j.molmet.2022.101634

Figure 8.

Figure 8

Patterns of 5-HT receptor subtype mRNA expression in ZIR of mice. A, Representative images showing 5-HT1a mRNA expression in the ZIR of mice with regular fed (A1), 24-h fasted (A2), and intermittent HFHS diet for 3 weeks (A3). Zoomed-in images were shown in the bottom left of each image. B, Representative images showing 5-HT2c mRNA expression in the ZIR of mice with regular fed (B1), 24-h fasted (B2), and intermittent HFHS diet for 3 weeks (B3). Zoomed-in images were shown in the bottom left of each image. C, Representative images showing 5-HT7 mRNA expression in the ZIR of mice with regular fed (C1 and zoomed-in image C4), 24-h fasted (C2 and zoomed-in image C5), and intermittent HFHS diet for 3 weeks (C3 and zoomed-in image C6). D, Bar graphs with data plots showing both 5-HT1a density (D1) and intensity (D2) in ZIR of mice with regular fed (n = 16 ZIR areas from slices of 3 mice), 24-h fasted (n = 16 ZIR areas from slices of 3 mice, and intermittent HFHS diet for 3 weeks (n = 16 ZIR areas from slices of 3 mice). Treatment effect for mRNA density: F(2,45) = 0.076, p = 0.9267. Treatment effect for mRNA intensity: F(2,45) = 0.218, p = 0.8052, ns, no significance. E, Bar graphs with data plots showing both 5-HT2c density (E1) and intensity (E2) in ZIR of mice with regular fed (n = 24 ZIR areas from slices of 4 mice), 24-h fasted (n = 14 ZIR areas from slices of 3 mice, and intermittent HFHS diet for 3 weeks (n = 24 ZIR areas from slices of 4 mice). Treatment effect for mRNA density: F(2,59) = 0.274, p = 0.7610. Treatment effect for mRNA intensity: F(2,59) = 0.990, p = 0.3773, ns, no significance. F, Bar graphs with data plots showing both 5-HT7 density (F1) and intensity (F2) in ZIR of mice with regular fed (n = 15 ZIR areas from slices of 3 mice), 24-h fasted (n = 10 ZIR areas from slices of 2 mice, and intermittent HFHS diet for 3 weeks (n = 14 ZIR areas from slices of 3 mice). Treatment effect for mRNA density: F(2,37) = 6.807, p = 0.003. Treatment effect for mRNA intensity: F(2,37) = 6.612, p = 0.004, ∗p < 0.05, ∗∗p < 0.01, ns, no significance.