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. 2022 Oct 4;15(1):13–37. doi: 10.1016/j.jcmgh.2022.09.015

Figure 5.

Figure 5

Expression of PTK7 and SOX9 in HCC cell lines. (A) A comparison of the expression of endogenous PTK7, SOX9, and secretory PTK7 in a panel of HCC cell lines. Coomassie Blue staining was used as a loading control for total secretory protein. (B) Quantitative PCR analysis on PTK7 messenger RNA (mRNA) level relative to that of the housekeeping gene β-actin across the HCC cell line panel. (C) Flow cytometry performed to evaluate the membrane expression of PTK7 across the HCC cell line panel. A mouse APC-conjugated IgG2a isotype control was used as the negative control. (D) A heterogenous population of PTK7-expressing and nonexpressing cells in PLC/PRF/5 as determined by flow cytometry. The 20% subsets with the highest- or lowest-membrane PTK7 expression were sorted out for functional and molecular assays. FSC, forward scatter.