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Primers Used in This Study
| Target | Sequence | Target size, base pair |
|---|---|---|
| qPCR | ||
| Human PTK7 | Forward: GTAGTAGCGAGGTATGAGGAGG Reverse: TGCGGTTAGTGATGGGAGTCT |
106 |
| Human SOX9 | Forward: AGCGAACGCACATCAAGAC Reverse: CTGTAGGCGATCTGTGGGG |
85 |
| Human ACTB (β-actin) | Forward: CATCCACGAAACTACCTTCAACTC Reverse: GAGCCGCCGATCCAGACG |
213 |
| For ChIP-qPCR | ||
| SOX9-binding site on PTK7 promoter | Forward: ATTCTGGGGGAACTGTTGGC Reverse: AGTGCAGGGGACCTGAGTAT |
202 |
qPCR, quantitative PCR.
