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. 2022 Nov 9;30:451–464. doi: 10.1016/j.omtn.2022.10.022

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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The efficiency of electroporation is increased by adding autophagy inhibitor 3-MA in the testes of 8-week-old mice

(A) The expression levels of LC3 in the testes of 4- and 8-week-old mice. GAPDH served as a loading control. (B) Western blot analysis of testis extracts after electroporation. Actin served as a loading control. (C) Western blot analysis of the expression levels of LC3 and p62 in mouse testes after adding autophagy inhibitor 3-MA and electroporation. Actin served as a loading control. (D) The expression levels of LC3 are decreased in mouse testes that included an autophagy inhibitor 3-MA after electroporation. Scale bar, 5 μm. (E) Western blot results showed that the expression levels of exogenous RNF20 were increased after adding autophagy inhibitor 3-MA in mouse testes at different time points after electroporation. Actin served as a loading control.