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. 2022 Nov 9;30:451–464. doi: 10.1016/j.omtn.2022.10.022

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Infertility caused by RNF20 depletion could be bypassed by electroporation coupled with ICSI

(A) Representative images of oocytes at 6 h after ICSI. Scale bar, 20 μm. (B) Statistical analysis of pronucleus formation of wild-type (totaling 81 injected oocytes) and Stra8-Rnf20^−/− + RNF20 (totaling 125 injected oocytes) mice. n = 3 biological replicates. Data are presented as mean ± SEM. n.s., non-significant. Statistical analysis was performed with two-tailed unpaired Student’s t test. (C) The average litter size of WT and Stra8-Rnf20^−/− + RNF20 mice. n = 3 biological replicates. Data are presented as mean ± SEM. n.s., non-significant. Statistical analysis was performed with two-tailed unpaired Student’s t test. (D) Representative images of Stra8-Rnf20^−/− offspring resulting from electroporation coupled with ICSI. (E) Representative genotypes of the Stra8-Rnf20^−/− offspring resulting from electroporation coupled with ICSI.