Fig. 2.

Endothelial NLRP3 inflammasome formation in the carotid arteries of endothelium-specific Smpd1 transgenic mice during hypercholesterolemia. A: Representative fluorescent confocal microscope images displaying the yellow dots or patches showing the colocalization of NLRP3 (green) with ASC (red). B: The summarized data showing the colocalization coefficient of NLRP3 with ASC. C: Representative fluorescent confocal microscope images showing the colocalization of NLRP3 (green) with caspase-1 (red). D: The summarized data show the colocalization coefficient of NLRP3 with caspase-1. Data are expressed as means ± SEM, n = 5. ∗P < 0.05 is defined as significant. The scale bar represents 20 μm or 200 μm.