Fig. 4.

Accumulation of ASM and ceramide in endothelial membrane rafts on the carotid artery wall of EC-specific Smpd1 transgenic mice during hypercholesterolemia. A: Representative fluorescent confocal microscopic images showing the colocalization of MR marker, flotillin (green) with ASM (red). B: Summarized data showing the colocalization coefficient of flotillin with ASM. C: Representative fluorescent confocal microscopic images showing the colocalization of MR marker, flotillin (green) with ceramide (red). D: Summarized data showing the colocalization coefficient of flotillin with ceramide. Data are expressed as means ± SEM, n = 5. ∗P < 0.05 as defined is significant. The scale bar represents 20 μm or 200 μm.