Fig. 5.
NOX subunit aggregation and superoxide production in the carotid artery wall during hypercholesterolemia. A: Representative fluorescent confocal microscopic images showing the colocalization of MR marker, flotillin (green) with NOX subunit, gp91phox (red). B: Summarized data showing the colocalization coefficient of flotillin with gp91phox. C: Representative fluorescent confocal microscopic images showing the colocalization of flotillin (green) with NOX subunit, gp47phox (red). D: Summarized data showing the colocalization coefficient of flotillin with gp47phox. E: Representative merged images (DHE red fluorescence merged with transmission light) showing the O2•− production in situ in mouse carotid arteries. F: Summarized data showing the DHE fluorescence intensity. Data are expressed as means ± SEM, n = 5. ∗P < 0.05 is defined as significant. The scale bar represents 20 μm or 200 μm for A and C and 200 μm for E.