Fig. 7.

Effects of ASM blockade on NLRP3 inflammasome activation in the primary cultures of ECs from EC-specific Smpd1 transgenic mice. A: Representative Western blot gel documents showing the expression of cleaved caspase-1 in the primary cultures of carotid ECs from endothelium-specific Smpd1 transgenic mice. B: Summarized data showing the expression of cleaved caspase-1 in the primary cultures of carotid ECs from endothelium-specific Smpd1 transgenic mice. C: Summary data showing caspase-1 activity in the primary cultures of carotid ECs from endothelium-specific Smpd1 transgenic mice. D: Summarized data showing IL-1β production in the primary cultures of carotid ECs from endothelium-specific Smpd1 transgenic mice. Data are expressed as means ± SEM, n = 5. ∗P < 0.05 is defined as significant.