Fig. 8.

Formation of ASM-ceramide MR signaling platforms in the primary cultures of ECs from EC-specific Smpd1 transgenic mice. A: Representative fluorescent confocal microscopic images showing the colocalization of MR marker, flotillin (red) with ASM (green). B: Summarized data showing the colocalization coefficient of flotillin with ASM. C: Representative fluorescent confocal microscopic images showing the colocalization of MR marker, flotillin (red) with ceramide (green). D: Summarized data showing the colocalization coefficient of flotillin with ceramide. Data are expressed as means ± SEM, n = 5. ∗P < 0.05 is defined as significant. The scale bar represents 10 μm.