Fig. 9.

Aggregation and activation of NOXs in MR signaling platforms in the primary cultures of ECs from EC-specific Smpd1 transgenic mice. A: Representative fluorescent confocal microscopic images showing the colocalization of MR marker, CTXB (green) with NOX subunit, gp47^phox (red). B: Summarized data showing the colocalization coefficient of CTXB with gp47^phox. C: Representative fluorescent confocal microscopic images showing the colocalization of LR marker, CTXB (green) with NOX subunit, gp91^phox (red). D: Summarized data showing the colocalization coefficient of CTXB with gp91^phox. E: NOX-mediated O₂•⁻ production as measured by ESR spectrometry. Data are expressed as means ± SEM, n = 5. ∗P < 0.05 is defined as significant. The scale bar represents 10 μm.