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. 2022 Oct 1;15(1):121–152. doi: 10.1016/j.jcmgh.2022.09.014

Figure 1.

Figure 1

RBMY plays multiple oncogenic functions in HCC cell lines. Subcutaneous HCC xenograft nude mice models engrafted with (A) siRNA-transfected and (B) shRNA-transduced Huh-7 cells were established. The endogenous RBMY was depleted with (A) siRBMY or (B) shRBMY. (B) Restoration of RBMY was achieved by overexpression of HA-RBMY. The xenograft tumor growth in nude mice was measured and compared with the control groups, (A) siCtrl and (B) shCtrl. The silencing efficacy of RBMY was assessed by immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) represents a loading control. (C) Tumor volume determined the xenograft growth and is plotted as means ± SDs. Statistical significance was evaluated with the Student t test. (D) Soft agar colony formation of stable Huh-7 transfectants was reported as means ± SDs (Student t test). (E) Scratch wound healing assay showed the migratory ability of indicated cells. Migration distance was assessed and depicted as means ± SDs (Student t test). (F) Transwell invasion assay showed the invasive capacity of the indicated cells. Bar graph (means ± SDs, Student t test) represents the number of cells migrating across Transwell membranes. RBMY-p, phosphorylated-RBMY.