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. 2022 Oct 1;15(1):121–152. doi: 10.1016/j.jcmgh.2022.09.014

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

ROS generation is crucial for PIM1/HA-RBMY-mediated mitochondrial motility and cellular migration/invasion. (A) PLC/PRF/5 cells stably expressing HA-PIM1 and/or HA-RBMY were treated with 30 μmol/L NAC and 1× PBS (mock) as solvent control. The distribution of mitochondria of these live cells were visualized by staining with Mito-ID Green. Staining of 4′,6-diamidino-2-phenylindole (DAPI) indicates the nucleus. Bar plots (means ± SDs) showed the distance of mitochondrial movement. The statistical significance was evaluated by the Student t test. (B) Scratch wound healing assay showed the migratory ability of cells indicated in the absence and presence of NAC. The migration distance was calculated and presented as means ± SDs. The Student t test was applied for the statistical analysis of the results. (C) Transwell invasion assay assessed the invasive potential of cells indicated with or without NAC treatment. The number of invaded cells was counted (means ± SDs) and the result between groups was compared by the Student t test. ∗P < .05, ∗∗P < .005, and ∗∗∗P < .0005.