Table 1.
Detailed Information of Antibodies Used in IHC and IFA Analysis
Antibody (clone/catalog number) | Manufacturer | Host species | Dilution and incubation conditions |
---|---|---|---|
RBMY (SRGY clone 1) | Homemade | Mouse | 5×, 4°C, Overnight (O/N) |
RBMY1A1 (GTX110098) | GeneTex | Rabbit | 150×, 4°C, O/N |
Her Par-1 (OCH1E5, ab75677) | Abcam | Mouse | 1×, 4°C, O/N |
PIM1 (TA354029) | OriGene | Rabbit | 150×, 4°C, O/N |
COX4 (GTX101499) | GeneTex | Rabbit | 100×, 4°C, O/N |
c-MET (8198) | Cell Signalling Technology | Rabbit | 100×, 4°C, O/N |
Fibronectin (GTX112794) | GeneTex | Rabbit | 100×, 4°C, O/N |
E-cadherin (ab1416) | Abcam | Mouse | 50×, 4°C, O/N |
Snail1 (sc-271977) | Santa Cruz Biotechnology | Mouse | 50×, 37°C, 2 h |
GRP94 (GTX103232) | GeneTex | Rabbit | 100×, 4°C, O/N |
Na+/K+-ATPase α (sc-48345) | Santa Cruz Biotechnology | Mouse | 500×, 4°C, O/N |
GOLPH2 (GTX107702) | GeneTex | Rabbit | 100×, 4°C, O/N |
CK19 (TA313117) | OriGene | Rabbit | 100×, 4°C, O/N |
HNF4α (C11F12) | Cell Signalling Technology | Rabbit | 100×, 4°C, O/N |
HA.11 epitope tag (901501) | BioLegend | Mouse | 100×, 4°C, O/N |