Table 2.
Information of the Antibodies Used in Western Blot Analysis
| Antibody (clone/catalog number) | Manufacturer | Host | Blotting conditions | Molecular weight, kilodaltons |
|---|---|---|---|---|
| RBMY (SRGY clone 1) | Homemade | Mouse | 10×, room temperature, 2 h | 56–68 |
| RBMY1A1 (GTX110098) | GeneTex | Rabbit | 1000×, 4°C, Overnight (O/N) | 56–68 |
| His (MAB050H) | R&D Systems | Mouse | 1500×, 4°C, O/N | Depends |
| GFP (PT-LA-GFP-RB) | Protech | Rabbit | 1000×, 4°C, O/N | Depends |
| HA.11 Epitope Tag (901501) | BioLegend | Mouse | 1000×, 4°C, O/N | Depends |
| GAPDH (GT239) | GeneTex | Mouse | 2000×, room temperature, 2 h | 38 |
| Lamin B1 (ab16048) | Abcam | Rabbit | 5000×, room temperature, 2 h | 68 |
| Lamin A/C (ab108922) | Abcam | Rabbit | 5000×, room temperature, 2 h | 74 |
| α-Tubulin (GTX115141) | GeneTex | Rabbit | 5000×, room temperature, 2 h | 55 |
| PIM1 (2907) | Cell Signalling Technology | Rabbit | 200×, 4°C, O/N | 34, 44 |
| Bcl2 (GTX100064) | GeneTex | Rabbit | 500×, 4°C, O/N | 26 |
| Bad (GTX130108) | GeneTex | Rabbit | 500×, 4°C, O/N | 22 |
| COX4 (GTX101499) | GeneTex | Rabbit | 1000×, 4°C, O/N | 16 |
| Snail1 (sc-271977) | Santa Cruz Biotechnology | Mouse | 100×, 37°C, 2 h | 29 |
| Slug (9585) | Cell Signalling Technology | Rabbit | 1000×, 4°C, O/N | 30 |
| Twist1 | GeneTex | Rabbit | 1000×, 4°C, O/N | 29 |
| ZEB1 (ab203829) | Abcam | Rabbit | 500×, 4°C, O/N | 210 |
| E-cadherin (ab1416) | Abcam | Mouse | 50×, 4°C, O/N | 110 |
| N-cadherin (GTX127345) | GeneTex | Rabbit | 500×, 4°C, O/N | 140 |
| Fibronectin (GTX112794) | GeneTex | Rabbit | 1000×, 4°C, O/N | 263 |
| Vimentin (ab16700) | Abcam | Rabbit | 100×, 4°C, O/N | 53 |
| Drp1 (ab184247) | Abcam | Rabbit | 1000×, 4°C, O/N | 83 |
| OPA1 (sc-393296) | Santa Cruz Biotechnology | Mouse | 100×, 4°C, O/N | 80-100 |
| Mfn2 (ab56889) | Abcam | Mouse | 1000×, 4°C, O/N | 86 |
| β-actin | Abcam | Rabbit | 2000×, 4°C, O/N | 45 |