FIG. 2.

Expression and localization of EGFP and 85B-EGFP fusion protein in vivo. Tibialis anterior muscles of C57BL/6 mice were injected with pEGFP, p65K-EGFP, or p85B-EGFP. Four days later, the mice were euthanized, and frozen sections of the muscle were visualized by fluorescence microscopy. EGFP and 65K-EGFP (not shown) produced homogenous fluorescence, but 85B-EGFP produced a speckled pattern. The clear areas inside the muscle cells are frozen-section artifacts.