Spleen weights (A) and spleen CFU (B) after M. avium infection in vaccination experiment 1. C57BL/6 mice were vaccinated with three intramuscular injections of saline, p65K-EGFP, or pCMV4.65 or with one intradermal injection of BCG vaccine and then challenged with intraperitoneal M. avium, as described in Materials and Methods. All groups contained five mice, and all data represent means ± standard errors. Each experimental group was compared to the saline group by using a two-tailed t test. NS, not significant.