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. 2022 Nov 16;32:101382. doi: 10.1016/j.bbrep.2022.101382

Fig. 3.

Fig. 3

Engraftment of transplanted PHs.

a. GFP-immunostaining in the liver of Auto model. Representative histological images of GFP-immunostained liver sections from the mice sacrificed at each time point (time after PH transplantation) are presented. From left to right, the liver images of the mice 1, 2, 4, 8 weeks after transplantation, respectively. 2 × objective's scale bar: 200 μm, 20 × objective's scale bar: 20 μm

b. Average values (%) of hepatocyte replacement indecies (RIs). GFP-positive areas of the tissue samples were calculated using ImageJ. All the data are expressed as mean ± SD. *p < 0.05. n = 3 in each group.

c. Hepatic GFP mRNA expression in Auto and Allo models. Hepatic mRNA expression levels were measured by qRT-PCR. Expression levels of the respective mRNAs were normalized to that of GAPDH and the data were presented as fold change compared with the average of Auto model at 1 week after PH transplantation. All the data are expressed as mean ± SD. *p < 0.05 n = 3 in each group.