Fig. 7.

Cognitive impairments are rescued by exogenous expression of GCH1 C141A mutant (MT-GCH1) in mice fed with homocysteine thiolactone (HTL). The experimental protocols were described and graphical in Online Fig. S5A. (A and B) Before sacrificed, MWM test was performed to determine spatial learning and memory in A. MCA flow velocity was determined by TCD in B. Quantitative analyses about MWM test, MCA flow velocity, and MCA pulse wave velocity were summarized in Table 4. (C and D) Left common carotid arteries isolated from mice were used for IHC analysis of 3-nitrotyrosine (3-NT) and HE staining in C. Quantitative analyses of 3-NT were shown in D. N = 10–15 per group. *P < 0.05 vs. WT-GCH1. ^#P < 0.05 vs. WT-GCH1 plus HTL. A one-way ANOVA followed by Scheffe test was used to determine P value between two groups in D.