Table 2.
Effects of NAC on antioxidative capacity in the ovaries.
| Item | Time (d) | Groups |
P value | |||
|---|---|---|---|---|---|---|
| CON | CN | HS | HSN | |||
| MDA content (nmol/mg prot) | 7 | 0.835±0.021 | 0.808±0.029 | 1.311±0.031***,# | 1.018±0.066* | <0.001 |
| 14 | 0.797±0.036 | 0.830±0.054 | 1.521±0.062***,### | 1.078±0.061⁎⁎ | <0.001 | |
| 21 | 0.942±0.045 | 0.868±0.066 | 1.598±0.053***,### | 1.078±0.051 | <0.001 | |
| CAT activity (U/mg prot) | 7 | 8.347±0.338 | 8.210±0.379 | 5.698±0.469**,# | 7.025±0.432* | 0.003 |
| 14 | 8.555±0.511 | 9.515±0.334 | 7.162±0.316*,# | 8.548±0.402 | 0.020 | |
| 21 | 9.687±0.299 | 9.509±0.369 | 4.967±0.207***,## | 7.821±0.521* | <0.001 | |
| SOD activity (U/mg prot) | 7 | 193.4±9.0 | 182.0±8.3 | 127.5±5.6***,## | 163.0±5.6⁎⁎ | 0.001 |
| 14 | 171.7±4.4 | 162.5±6.0 | 117.5±7.3**,## | 161.4±7.8 | <0.001 | |
| 21 | 174.2±5.2 | 185.3±8.4 | 108.5±4.6***,## | 156.1±7.2 | <0.001 | |
| GSH-Px activity (U/mg prot) | 7 | 257.4±11.8 | 254.1±10.3 | 158.4±10.0**,# | 200.8±8.4⁎⁎ | <0.001 |
| 14 | 213.0±8.7 | 231.1±11.4 | 129.6±11.7***,# | 171.2±11.5* | 0.001 | |
| 21 | 234.6±7.3 | 229.6±7.7 | 135.1±11.6***,# | 180.6±7.8⁎⁎ | <0.001 | |
| T-AOC activity (U/mg prot) | 7 | 2.291±0.082 | 2.366±0.086 | 1.683±0.044⁎⁎⁎ | 1.761±0.054⁎⁎⁎ | <0.001 |
| 14 | 2.173±0.143 | 2.110±0.105 | 1.349±0.075***,## | 1.860±0.078 | 0.001 | |
| 21 | 2.068±0.067 | 2.148±0.101 | 1.286±0.056***,## | 1.739±0.067* | <0.001 | |
Each value represents the mean ± SE of six growing pullets per group.
Abbreviations: CAT, catalase; CON, control group; CN, control+1 g/kg NAC group; GSH-Px, glutathione peroxidase; HS, chronic heat stress group; HSN, chronic heat stress +1 g/kg NAC group; MDA, malondialdehyde; SOD, superoxide dismutase; T-AOC, total antioxidant capacity.
“*”Indicates a significant difference compared to the corresponding control: *P < 0.05, **P < 0.01., ***P < 0.001.
“#”Indicates a statistically significant difference between the corresponding groups: #P < 0.05; ##P < 0.01; ###P < 0.001.