Figure 4.
Results from the fluorescence-based OM permeabilization assay of MRL-494 (1) and analogues 13, 16, and 17 against E. coli BW25113. Fluorescence of N-phenylnaphthalen-1-amine (NPN) was read using a plate reader with λex = 355 nm and λem = 420 nm after 60 min of incubation. The NPN uptake values shown are calculated relative to the uptake obtained when the cells are treated with colistin (100 μg/mL). The values are also corrected for the background signal determined by the negative control (DMSO). Error bars represent the standard deviation based on technical replicates (n = 3).