Figure 6.

Real-time monitoring of bacterial growth and Rcs stress activation in response to MRL-494 (1) and analogues 13, 16, and 17. E. coli TOP10F′ cells, harboring the PrprA-mNG reporter construct, were grown in a 96-well plate and exposed to the compounds at the indicated concentration at time point 0. Growth (A; OD₆₀₀) and mNG fluorescence (B) were measured in time. Fluorescence was corrected for growth (OD₆₀₀) and plotted as fold-change of signal compared to untreated cells (set to 1). Error bars represent the standard deviation of triplicate technical replicates.