Table 2. Photophysical Characteristics of New Derivatives^a.

conjugate	εDMF, M–1 cm–1	buffer	Abs (λmax) [3 μM]	Ip (λmax) [0.5 μM]	Ip/Abs [0.5 μM]	LT (μs), 37 °C	QY, Φ
PtPFPP	257 000b	PBS + 1% TX-100			1593	60c (CH2Cl2)	0.088c (CH2Cl2)
		PBS + 1% TX-100 + sulfite			16 937
Pt1Glc4	227 100d	PBS		56 (657)	956	9.1	0.0050
		PBS–sulfite		71 (656)	1528	30.7	0.0079
		PBS + 5% FBS		829 (650)	9261	15.5	0.0481
		PBS + 5% FBS + sulfite		1353 (652)	17 685	37.3	0.0919
Pt2Glc4	256 200	PBS	0.32 (404)	22 (658)	691	9.2	0.0036
		PBS–sulfite		26 (657)	1209	20.2	0.0063
		PBS + 5% FBS	0.36 (400)	126 (653)	2184	14.3	0.0113
		PBS + 5% FBS + sulfite		546 (652)	8312	36.7	0.0432
PtcPEG4	291 100	PBS	0.28 (392)	35 (653)	705	8.1	0.0037
		PBS–sulfite		90 (653)	2152	25.9	0.0112
		PBS + 5% FBS	0.31 (396)	288 (651)	4878	11.7	0.0253
		PBS + 5% FBS + sulfite		559 (651)	11 599	36.1	0.0603
PtcPEG31Glc	222 400	PBS	0.4 (392)	44 (652)	546	8.2	0.0028
		PBS–sulfite		91 (651)	1320	31.0	0.0069
		PBS + 5% FBS	0.54 (396)	486 (651)	4904	11.7	0.0255
		PBS + 5% FBS + sulfite		747 (650)	8379	34.7	0.0435
PtCA31Glc	230 700	PBS	0.34 (395)	15 (656)	250	11.0	0.0013
		PBS–sulfite		24 (661)	443	34.8	0.0023
		PBS + 5% FBS	0.44 (395)	229 (651)	3025	16.6	0.0157
		PBS + 5% FBS + sulfite		506 (652)	6399	38.6	0.0332

^aPBS = phosphate buffer saline. FBS = fetal bovine serum. 10% of sulfite (5 mg/mL KH₂PO₄, 5 mg/mL Na₂SO₃) was added to deoxygenate the buffer and measure the corresponding lifetime values in air-saturated and deoxygenated conditions. Molar extinction coefficients (ε) were calculated according to the Lambert–Beer Law. Relative quantum yields (Φ) were calculated in relation to PtPFPP. Absorption and emission of the reference dye were measured in deoxygenated aqueous media, assuming its quantum yield as 0.088.

^bReference (39).

^cReference (40).

^dReference (22).