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. 2022 Nov 18;29:98. doi: 10.1186/s12929-022-00882-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — rCD248 inhibits experimental lung metastasis in mice. Experimental lung metastasis assay. A The experimental protocol of mouse model of lung metastasis assay. Twenty µg of rCD248D1-4 protein was given 1 h before and 1 and 2 days after intravenous inoculation of B16F10 cells into each mouse. B Representative pictures of the gross view of mouse lung surface 14 days after tumor inoculation. C Statistical analysis of lung surface occupation of tumor nodules. N = 9 for the control group (Ctrl) and N = 8 for the rCD248D1-4 group. ***, P < 0.001. D Representative CD248 expression patterns in mouse lung isolated from normal mice and mice after experimental metastasis assay. E Representative images of the CD31 (brown color) and PAS (magenta color) double stain in the lung tumor nodules. The PAS⁺/CD31⁻ area is denoted as vascular mimicry (VM) phenotype. F Statistical analysis of VM channel (PAS⁺/CD31⁻ stain) in the lung tumor nodule. **P < 0.01. N = 20 nodules for the Ctrl group and N = 21 nodules for the rCD248D1-4 group. G Representative images of MMP9 stain in the lung tumor nodules and H the statistical analysis thereof. ***P < 0.001. N = 117 nodules for the Ctrl group and N = 69 nodules for the rCD248D1-4 group