Table 2.
Detection of EBV genome by applying conventional (EBER-ISH) and unconventional methods (ddPCR, RNAscope) in two different histological types of GC (ADK, GCLS)
| Case Number | Assay | Histological Type | |||
|---|---|---|---|---|---|
| EBER-ISH | ddPCR | RNAScope | |||
| EBERs | BamHI (copies/ul) | EBNA1 (copies/ul) | EBNA1 mRNAs (score) | ||
| #1 | − | 2 | 0 | – | ADK |
| #2 | − | 0 | 0 | – | |
| #3 | + | 988 | 1287 | 2500 | |
| #4 | − | 0 | 0 | – | |
| #5 | − | 0 | 0 | – | |
| #6 | − | 0 | 0 | – | |
| #7 | − | 0 | 0 | – | |
| #8 | − | 0 | 0 | – | |
| #9 | − | 0 | 0 | – | |
| #10 | − | 0 | 0 | – | |
| #11 | − | 2.2 | 0 | – | |
| #12 | − | 9.8 | 1.5 | 8 | |
| #13 | − | 0 | 0 | – | |
| #14 | − | 0 | 0 | – | |
| #15 | − | 1.4 | 0 | 6 | |
| #16 | − | 0.3 | 0 | – | |
| #17 | − | 0.5 | 0 | – | |
| #18 | − | 0 | 0 | – | |
| #19 | − | 0 | 0 | – | |
| #20 | − | 0 | 0 | – | |
| #21 | − | 1.5 | 0.9 | 8 | |
| #22 | − | 0 | 0 | – | |
| #23 | − | 0 | 0 | – | |
| #24 | − | 5.7 | 0 | 7 | |
| #25 | − | 0 | 0 | – | |
| #26 | − | 0 | 0 | – | |
| #27 | − | 0 | 0 | – | |
| #28 | + | 1202 | 1314 | 2600 | |
| HCL | 0 | 0 | |||
| HCL | 0 | 0 | |||
| HCL | 0 | 0 | |||
| #29 | − | 0.52 | 0.17 | – | GCLS |
| #30 | − | 41.85 | 4 | 10 | |
| #31 | − | 19.1 | 2.03 | 8 | |
| #32 | − | 3.23 | 0.53 | 12 | |
| #33 | − | 0.94 | 0.48 | – | |
| #34 | − | 0.46 | 0.18 | – | |
| #35 | − | 0 | 0 | – | |
| #36 | + | 6460 | 2847 | 3000 | |
| #37 | + | 1410 | 5100 | 2900 | |
| #38 | + | 2710 | 1351 | np | |
| #39 | + | 466 | 152 | np | |
| #40 | + | 2089 | 587 | np | |
| HCL | 0 | 0 | |||
| HCL | 0 | 0 | |||
| HCL | 0 | 0 | |||