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. 2022 Nov 17;17:33. doi: 10.1186/s13062-022-00347-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Silencing of SH3BP5-AS1 expression inhibited the invasion, migration, and stemness of pancreatic cancer cells in vitro. vivo. A, B Transwell assay revealed the migration and invasion abilities of BxPC-3 and MIA Paca-2 cells transfected with shCtrl or shSH3BP5-AS1. C, D Histogram displaying the statistical results of the Transwell assay. E EMT-related markers were detected by western blot. F–I Sphere formation analysis. J–L Stem cell-like cell markers Lin28, OCT4, NANOG, and SOX-2 were detected by western blot and RT-qPCR. Data are shown as the mean ± SD of three replicates. *P < 0.05; **P < 0.01