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. 2022 Nov 17;53:95. doi: 10.1186/s13567-022-01109-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Biofilm formation ability of M. hyopneumoniae strains. A–F Biofilms formed by strain NJ, which were stained with crystal violet and observed under a microscope after A 1 day, B 2 days, C 3 days, D 4 days, and E 5 days of incubation. F negative control. G, H Comparison of biofilms formed by seven M. hyopneumoniae strains. Biofilm formation ability of strains was determined and identified by crystal violet staining after 4 days of incubation. Bars represent mean ± SD for three independent replicate experiments. KM2 represents control (uninoculated broth).