Fig. 3.
Combined DCX/MET treatment induces metabolic decoupling in PC-3 WT prostate cancer cells. A–C PC-3 WT and PC-3_DCX20 cells were subjected to DCX and/or MET (2.5 nM/10 mM; 48 h). Metabolic activity (MTT) (A), ATP content (B), and lactate production (C) were analyzed and calculated per 105 cells. D Cells were incubated as in A, and drug efflux efficiency was measured with a calcein efflux assay. The statistical significance of the differences was tested with Student’s t-test (A–D). #P ≤ 0.05 versus untreated control; *P ≤ 0.05 as indicated in the charts or PC-3 WT in D. All results are representative of at least three independent experiments (N ≥ 3). Error bars represent SEM. Note relatively low MTT/ATP levels and negligible impairment of calcein efflux (metabolic decoupling) in PC-3_DCX20 cells