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. 1999 Aug;67(8):4272–4275. doi: 10.1128/iai.67.8.4272-4275.1999

FIG. 4.

FIG. 4

PCR analysis of FnBP gene fragments. (A) Genomic DNA from S. aureus 8325-4 (lane 3) and S. schleiferi NCTC 12218 (lane 4) was amplified with primers complementary to the 345-bp D1 to D3 binding domain of S. aureus 8325-4 fnbA. Lane 1, 500-bp molecular size marker; lane 2, 100-bp molecular size marker. (B) Map showing the possible positions at which the fnbA primers could anneal in the S. aureus 8325-4 fnb locus (F, forward primer; R, reverse primer). Regions marked S, A, B, C, D, W, and M are domains in the FnBPA and FnBPB proteins.