Table 1.

IC₅₀ values of compound 6 and novel N-acyl amino acid conjugates 7a–m in different cell lines.

Compounds	IC50 (µM)
	HeLa	PC-3	HCT-116	BxPC-3	HepG-2	MCF-7	DHFR	HPDE
6	15.11 ± 1.21	14.91 ± 1.24	17.26 ± 2.18	14.21 ± 1.36	12.98 ± 1.86	11.91 ± 1.10	7.99 ± 0.16	>50
7a	19.45 ± 1.21	23.03 ± 1.03	28.24 ± 1.93	19.45 ± 1.56	15.45 ± 0.96	15.29 ± 1.29	13.27 ± 0.24	>50
7b	29.21 ± 1.90	34.24 ± 2.61	29.56 ± 1.91	31.23 ± 2.80	19.23 ± 1.87	35.67 ± 2.09	42.49 ± 0.78	>50
7c	9.39 ± 0.25	11.25 ± 0.34	10.60 ± 1.10	10.25 ± 1.01	12.25 ± 1.14	6.56 ± 0.18	0.61 ± 0.01	>50
7d	8.09 ± 0.51	9.99 ± 0.81	10.09 ± 1.01	11.19 ± 0.41	9.09 ± 0.71	5.51 ± 0.28	0.43 ± 0.01	>50
7e	13.11 ± 1.01	13.81 ± 1.14	15.26 ± 1.18	15.21 ± 1.15	15.98 ± 0.80	11.91 ± 1.10	1.83 ± 0.04	>50
7f	6.99 ± 1.11	6.29 ± 0.56	5.48 ± 0.04	7.09 ± 0.09	5.12 ± 0.01	4.65 ± 0.17	0.31 ± 0.01	>50
7g	12.29 ± 1.20	13.21 ± 1.52	12.21 ± 1.20	15.21 ± 0.22	10.21 ± 0.92	10.78 ± 1.10	1.28 ± 0.03	>50
7h	18.01 ± 1.20	17.01 ± 2.02	19.34 ± 0.85	17.87 ± 1.03	16.99 ± 0.92	19.98 ± 2.01	8.47 ± 0.18	>50
7i	10.91 ± 1.99	12.78 ± 2.01	13.04 ± 1.01	10.54 ± 3.01	9.26 ± 1.56	13.21 ± 1.21	0.82 ± 0.02	>50
7J	9.29 ± 0.20	11.17 ± 0.70	8.10 ± 0.20	6.19 ± 0.43	10.29 ± 1.20	8.19 ± 1.20	0.54 ± 0.01	>50
7k	19.53 ± 1.10	19.88 ± 1.35	18.02 ± 1.23	19.08 ± 1.23	25.54 ± 0.14	28.90 ± 1.91	7.00 ± 0.17	>50
7l	9.05 ± 1.20	10.08 ± 0.90	12.99 ± 0.89	11.45 ± 0.76	13.19 ± 1.29	10.67 ± 1.09	0.66 ± 0.01	>50
7m	11.81 ± 1.13	12.72 ± 1.87	13.32 ± 2.21	11.99 ± 1.14	14.25 ± 0.98	11.30 ± 1.28	1.34 ± 0.03	>50
Doxorubicin	5.57 ± 0.51	9.01 ± 0.09	5.52 ± 0.13	7.23 ± 0.10	4.6 ± 0.44	4.25 ± 0.15	–	>50
Methotrexate	294.77 ± 0.51	42.33 ± 7.25	99.98 ± 0.22	29.11 ± 0.31	800.24 ± 0.16	66.11 ± 0.15	5.61 ± 0.11	>50