Fig. 2.

Effect of GAAE on DCFH2 & Alexa405 fluorescence.

DCFH2 and Alexa 405 yeast were incubated with GAAE (25 ng/ml; 250 ng/ml; 2,5 μg/ ml and 25 μg/ml) and 1 mM H₂O₂ for 30 minutes. Flow cytometry was used to examine yeast fluorescence with an excitation wavelength of 488 nm (A): DCF fluorescence of non-oxidized DCFH2-labeled yeast; blue line: DCFH2-labeled yeast fully oxidized by H₂O₂, green lines: DCFH2-labeled yeast oxidized by H₂O₂, in the presence of GAAE, the dark green line represents the highest GAAE concentration (25 μg/ml), and results were represented as fluorescence histograms at 405 nm (B). The black line represents non-labeled yeast, the blue line represents Alexa 405-labeled yeast, and the dotted blue line represents Alexa 405-labeled yeast following incubation with 25 μg/ml GAAE.