Figure 1 |. Reduced clearance of aberrant transcripts by NMD in tau transgenic Drosophila.

A) Panneuronal RNAi-mediated reduction of Smg5 limits NMD in Drosophila based on activation of an NMD sensitive reporter. n=6 biological replicates per genotype, per age. B) Panneuronal expression of transgenic human tau^R406W limits NMD in Drosophila at day one and day ten of adulthood based on activation of an NMD sensitive reporter. n=6 biological replicates per genotype, per age. Transcript levels of Gadd45 and Arc1 are significantly elevated in Drosophila with panneuronal RNAi-mediated reduction of Smg5 (C) and in tau^R406W transgenic Drosophila at day one and day ten of adulthood (D, E). F) Panneuronal expression of transgenic human tau^WT limits NMD in Drosophila at day twenty of adulthood based on activation of an NMD sensitive reporter. n=6 biological replicates per genotype. Panneuronal expression of tau^WT elevates Gadd45 (G) and Arc1 (H) transcript levels compared to controls. n=12 biological replicates per genotype. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, unpaired t-test and multiple unpaired t-test. Error bars=SEM. Full genotypes are included in Supplemental Table 1.