Figure 4 |. Experimentally elevating NMD decreases RNA accumulation in nuclear envelope invaginations in brains of tau^R406W transgenic Drosophila.

A) Upf1 RNA levels in tau^R406W Drosophila with and without Upf1 overexpression. B) Immunofluorescence-based quantification of Upf1 protein levels in tau^R406W Drosophila with and without Upf1 overexpression. C) ELISA-based NMD-sensitive reporter activity in control Drosophila with and without Upf1 overexpression. Gadd45 (D) and Arc1 (E) transcript levels based on dPCR in tau^R406W Drosophila with and without Upf1 overexpression. F) FISH/IF-based detection of polyA RNA and lamin in tau^R406W Drosophila with and without Upf1 overexpression. Representative images include an orthogonal view of zoom region depicted showing polyA RNA-positive and -negative invaginations. G) Quantification of (F), percentage of nuclear envelope invaginations containing polyA RNA in flies of the indicated genotype. All assays were performed at day ten of adulthood. n=6–12 biological replicates per genotype. p*<0.05, one-way ANOVA. Error bars=SEM. Full genotypes are included in Supplemental Table 1.