TABLE 2.
Comparative infectivity of P. berghei sporozoites to immunized CD-1 outbred mice when sporozoites were delivered by either infectious mosquito bite or syringe inoculation into mouse tail veina
| Immunizing agent | Host immunoglobulin G titer | % of mice infected after:
|
|||
|---|---|---|---|---|---|
| 5 Mosquito bites | 10 Mosquito bites | Intravenous inoculation of:
|
|||
| 20,000 spz | 50,000 spz | ||||
| Irradiated spz | >1:1,000 | 2 (41) | 43 (14) | 0 (20) | 0 (14) |
| Peptide-KLH | >1:3,200 | 83 (6) | ND | 0 (6) | ND |
| KLH only | <1:20 | 100 (19) | ND | ND | ND |
| None | <1:20 | ND | ND | ND | 100 (12) |
a
Mice were immunized against either irradiated sporozoites (spz) or the synthetic peptide D-16-N coupled to KLH. Negative-control mice received KLH only. Sample sizes are indicated within parentheses. ND, not done.