Table 2.
PCR primers for Filovirus L gene, Coronavirus RdRp region, Nipah Virus N gene, Rhabdovirus L gene and their expected amplicon size4,8,21
| Target | Primer | Sequence (5' to 3') | Annealing temperature | Size (base pair) |
|---|---|---|---|---|
| Filovirus L gene | FV-F1 | GCMTTYCCIAGYAAYATGATGG | 460 bp | |
| FV-R1 | GTDATRCAYTGRTTRTCHCCCAT | |||
| FV-F2 | TDCAYCARGCITCDTGGCAYC | 54 °C | 350 bp | |
| FV-R2 | GIGCACADGADATRCWIGTCC | |||
| Coronavirus RdRp region | IN-6 | GGTTGGGACTATCCTAAGTGTGA | 56 °C | 440 bp |
| IN-7 | CCATCATCAGATAGAATCATCATA | |||
| Nipah N gene | Forward | ATGCCATGGATCCCGAATGAGTGATAT TTTTGAAGAGGCGGCTAG | 51 °C | 1500 bp |
| Reverse | CGTAGCACTCGAGGATTCACACATCAG CTCTGACAAAGTCAAG | |||
| Rhabdovirus L gene | Forward | CCADMCBTTTTGYCKYARRCCTTC | ||
| Reverse | RAAGGYAGRTTTTTYKCDYTRATG | |||
| Forward 2 | ATGACAGACAACCTGAACAA | 58 °C | 340 bp | |
| Reverse 2 | CCATTCCAGCAGGTTGGACC |