Fig 1. Effects of mutations in Omicron BA.1 and BA.2 receptor-binding domains on ACE2-binding and RBD expression.

(A) Diagram of the RBD substitutions that distinguish Omicron BA.1 and BA.2 from Wuhan-Hu-1. We use Wuhan-Hu-1 spike numbering throughout. (B, C) Heatmaps illustrating the impacts of all single mutations on ACE2-binding affinity (B) and RBD surface expression (C), as determined from FACS-seq assays on yeast-displayed RBD mutant libraries. See S1–S3 Figs. for experimental details. Individual measurements are reported in S1 and S2 Data, and an interactive version of these heatmaps is available at https://jbloomlab.github.io/SARS-CoV-2-RBD_DMS_Omicron/RBD-heatmaps/.