Figure 1. Capicua represses YAP1.

(A) Heatmap of CIC peaks relative to transcription start sites (upstream −3 kb to downstream +3 kb) in HCC1359s.

(B) % of CIC peaks located at genomic regions.

(C) ChIP-seq track from HCC1359s demonstrating CIC occupancy (upper red) of a YAP1 RE. Immunoglobulin (Ig)G control (lower gray).

(D) Alignment of WT-CIC (HCC1359 and HOG) and CIC-DUX4 (NCC_CDS1_X1_C1) ChIP peaks. Genomic coordinates of peaks indicated with dashed lines.

(E) YAP1 RE with locations of ChIP-PCR primers (yellow bars).

(F–H) ChIP-PCR showing CIC occupancy on YAP1 in HCC1359 (F and G) (n = 3) and HEK293T (H) cells. *p < 0.05.

(I) Relative YAP1 expression in H1975, H1975_10.1, and H1975_10.1 expressing GFP-CIC. *p < 0.05.

(J) Relative YAP1 expression in H1975s expressing sgCtrl, sgCIC1, or sgCIC2. *p < 0.05, **p < 0.01.

(K) Relative YAP1 in BEAS-2Bs expressing scramble (siSCR) or siCIC. *p < 0.05.

(L–Q) Immunoblots (IB) of CIC, ETV4, and YAP in H1975 (L), HCC1359 (M), HCC364 (N), and HEK293T (O) expressing sgCtrl, sgCIC1, or sgCIC2. IB of CIC, ETV4, and YAP in HCC1359 (P) and HCC364 (Q) cells expressing control, CIC^WT, or CIC^ΔERKBD.

(R) IB of pERK, tERK, CIC, and YAP in HCC1359s treated with trametinib (1 μM, 24 h).

(S) Schematic of YAP/TAZ reporter.

(T) Relative YAP/TAZ reporter activity in HEK293Ts expressing sgCtrl, sgCIC1, or sgCIC2. **p < 0.01.

(U) Relative YAP/TAZ reporter activity in H1975 + EV, H1975_10.1 + EV, and H1975_10.1 + CIC expression. **p < 0.01.

Error bars represent SD.