| Antibodies |
|
| CIC |
Thermo fisher scientific |
PA1-46018; RRID: AB_2291736 |
| YAP |
Cell signaling technology |
Cat# 14074; RRID: AB_2650491 |
| LATS1 |
Cell signaling technology |
Cat# 3477; RRID: AB_2133513 |
| LATS2 |
Cell signaling technology |
Cat# 5888; RRID: AB_10835233 |
| Myc-tag |
Cell signaling technology |
Cat# 2276; RRID: AB_331783 |
| p44/42 MAPK (Erk1/2) |
Cell signaling technology |
Cat# 4695; RRID: AB_390779 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) |
Cell signaling technology |
Cat# 4370; RRID: AB_2315112 |
| HSP90 |
Cell signaling technology |
Cat# 4874; RRID: AB_2121214 |
| Normal Rabbit IgG |
Cell signaling technology |
Cat# 2729; RRID: AB_1031062 |
| ETV4 (PEA3) |
Santa Cruz Biotechnology |
Cat# sc-166629; RRID: AB_2278090 |
|
| Bacterial and virus strains |
|
| Stbl3™ Chemically Competent E. coli |
Thermo fisher scientific |
Cat# C737303 |
|
| Biological samples |
|
| Lung adenocarcinoma tissue array |
US Biomax |
Cat# LC10014a |
|
| Chemicals, peptides, and recombinant proteins |
|
| Trametinib |
MedChemExpress |
Cat# HY-10999 |
| Osimertinib |
MedChemExpress |
Cat# HY-15772 |
| Vemurafenib |
MedChemExpress |
Cat# HY-12057 |
| Verteporfin |
MedChemExpress |
Cat# HY-B0146 |
| Recombinant Human EGF |
Peprotech |
Cat# AF-100-15 |
| FuGENE6 transfection reagent |
Promega |
Cat# E2692 |
| Lipofectamine RNAiMax transfection reagent |
Thermo fisher scientific |
Cat# 13778150 |
| Blasticidin S HCl |
Thermo fisher scientific |
Cat# A1113903 |
| Puromycin Dihydrochloride |
Thermo fisher scientific |
Cat# A1113803 |
| T4 DNA ligase |
New England Biolabs |
Cat# M0202S |
| Phusion high-fidelity DNA polymerase |
New England Biolabs |
Cat# 0530L |
| XhoI |
New England Biolabs |
Cat# R0146S |
| KpnI-HF |
New England Biolabs |
Cat# R3142S |
|
| Critical commercial assays |
|
| ECL prime western blotting system |
Amersham |
Cat# RPN2232 |
| RNeasy mini kit |
Qiagen |
Cat# 74106 |
| DNeasy blood & tissue kit |
Qiagen |
Cat# 69504 |
| QIAGEN plasmid plus maxi kit |
Qiagen |
Cat# 12963 |
| QIAquick PCR purification kit |
Qiagen |
Cat# 28104 |
| Q5 Site-directed mutagenesis kit |
New England Biolabs |
Cat# E0554 |
| Applied biosystems Taqman ast advanced master mix |
Thermo fisher scientific |
Cat# 4444557 |
| SimpleChIP enzymatic chromatin IP kit (Magnetic beads) |
Cell signaling technology |
Cat# 9003 |
| Dual-luciferase reporter assay system |
Promega |
Cat# E1910 |
| CellTiter-Glo luminescent cell |
Promega |
Cat# G7572 |
|
| Deposited data |
|
| ChIP-seq data |
Yang et al., 2017
|
GSE95012 |
|
| Experimental models: Cell lines |
|
| Human embryonic kidney HEK293T |
ATCC |
CRL-3216; RRID: CVCL_0063 |
| Human lung adenocarcinoma H1975 |
ATCC |
CRL-5908; RRID: CVCL_1511 |
| Human lung adenocarcinoma H1975_10.1 |
Walter et al. (2013)
|
N/A |
| Human lung giant cell carcinoma HCC1359 |
ATCC |
N/A; RRID: CVCL_5128 |
| Human lung adenocarcinoma HCC364 |
ATCC |
N/A; RRID: CVCL_5134 |
| Human lung adenocarcinoma A549 |
ATCC |
CCL-185; RRID: CVCL_0023 |
| Human lung adenocarcinoma SW1573 |
ATCC |
CRL-2170; RRID: CVCL_1720 |
| Human malignant melanoma A375 |
ATCC |
CRL-1619; RRID: CVCL_0132 |
| Human colon adenocarcinoma HT-29 |
ATCC |
HTB-38; RRID: CVCL_0320 |
| Patient-derived CIC-DUX4 sarcoma NCC_CDS1_X1_C1 |
Oyama et al. (2017)
|
N/A |
|
| Experimental models: Organisms/strains |
|
| NU/J athymic female nude mice |
Jackson Laboratory |
Strain #:002019; RRID: IMSR_JAX:002019 |
|
| Oligonucleotides |
|
| Oligonucleotides for RT-qPCR, ChIP-PCR and luciferase promoter assay, see supplemental tables provided |
This study |
This study |
|
| Recombinant DNA |
|
| CIC (Myc-DDK-tagged) human capicua homolog |
Origene |
Cat# 215209 |
| Lenti-ORF clone of CIC (mGFP-tagged) human capicua homolog |
Origene |
Cat# 215209L2 |
| 8xGTIIC-luciferase |
Addgene |
Cat# 34615 |
| PXPR007 sgCIC-1 |
Addgene |
Cat# 74953 |
| PXPR007 sgCIC-2 |
Addgene |
Cat# 74959 |
| LV05 vector (U6-gRNA:EF1a-Cas9+FLAG-2A-Puro) containing sgLATS2 |
Sigma-aldrich |
N/A |
| YAP1 shRNA #2 |
A gift from T. Bivona |
Lin et al., 2015
|
| YAP1 shRNA #3 |
A gift from T. Bivona |
Lin et al., 2015
|
|
| Software and algorithms |
|
| GraphPad Prism 9.0 software |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/
|
| Image J |
National Institutes of Health |
https://imagej.nih.gov/ij/download.html
|
| MEME-ChIP (DREME, STREME, AME) |
Machanick and Bailey (2011)
|
https://meme-suite.org/meme/doc/meme-chip.html?man_type=web
|
| ZEISS ZEN 3.6 Imaging Software |
Zeiss |
https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html
|
| ABI Prism 7900HT Sequence Detection System |
Applied Biosystems |
https://www.bu.edu/picf/files/2017/06/ABI7900HT_usersguide.pdf
|
| Model-based Analysis of ChIP-Seq (MACS2) algorithm (version 2.2.1) |
N/A |
PMID:18798982 |
| R package ChIPseeker algorithm |
N/A |
PMID:25765347 |
| DAVID pathway analysis |
N/A |
https://david.ncifcrf.gov
|
