Fig. 1.

Integration of automated screening platform. (A) Schematics of the automated screening platform for cancer immunotherapy screening, consisting of (i) a microfluidic immune-tumor interaction platform for high-throughput tracking of T cell dynamic behaviors and (ii) a deep learning–based TIL score analyzer for image processing and scoring T cell infiltration patterns that correspond to TIL patterns found in the images of high-survival (>3 y) or low-survival patient groups. (B) Scalable microfluidic fabrication of core/tumor-shell/stroma spheroids (tumor core: blue, CAF; shell: yellow). (C) Spheroid growth within pillar-lattice-arrays (arrow indicating CAF seeding after tumor core formation), and size distribution of tumor-stroma spheroids at day 4 of spheroid formation. (Scale bar in B: 500 µm.)