Table 1. Summary of the antibody and microscope settings used for immunofluorescence imaging.

Target	Primary antibody	Secondary antibody	Microscope excitation
PD1	Rat anti-mouse PD1 (100 mg/kg delivered in vivo; BP0146, Bio X Cell)	Goat anti-rat Alexa Fluor 647 (A21247, Invitrogen)	639 nm
CD31	Rabbit anti-mouse CD31 (ab28364, Abcam Inc.)	Goat anti-rabbit Alexa Fluor 488 (A11008, Invitrogen)	488 nm
ICAM	Rat anti-mouse ICAM-1 (14-0542-85, Invitrogen)	Goat anti-rat Alexa Fluor 647 (A21247, Invitrogen)	639 nm
Macrophages/microglia	Rabbit anti-mouse Iba1 (019-19741, Fujifilm, Wako Chemicals)	Donkey anti-rabbit Alexa Fluor 555 (A31572, Invitrogen)	555 nm
DAPI	DAPI solution (62248, Invitrogen)		405 nm