Fig. 2. Mitochondrial nonstop mRNAs are generated in health and disease.
(A and B) Deep sequencing analysis of the 3′ end of the mitochondrial mRNAs MT-CO3, CYTB, ND1, ND2, ND3, and ND4 that do not encode a stop codon and require tRNA processing followed by polyadenylation to generate a stop codon. RNA isolated from mitochondrial ribosomes in cultured fibroblasts with the indicated genotypes. CDS + poly A, polyadenylated CDS. WT, wild type. (C) Total abundance of mitochondrial nonstop mRNAs sequenced from MT-CO3, CYTB, ND1, ND2, ND3, and ND4 transcripts in the total transcriptome versus isolated from mitochondrial ribosomes. Each data point represents an independent sample and library preparations.