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. 2022 Sep 21;20(12):2406–2417. doi: 10.1111/pbi.13922

Figure 1.

Figure 1

Generation of overexpression (OE) plants and knockout mutants of BnaBASS2. (a) Gene expression of BASS2s in Brassica napus tissues. At, Arabidopsis thaliana; Bn, B. napus; Gene expression data are from BnTIR (http://yanglab.hzau.edu.cn/). (b) BnaA05.BASS2 is a chloroplast membrane protein by the observation of tobacco epidermal cells under confocal. Bars = 17 μm. (c) Relative expression level of BnaA05.BASS2 in OE lines by quantitative real‐time PCR. Total RNA was extracted from 24 DAF seeds. Values are means ± SD (n = 3). ** indicates P < 0.01, based on a student t‐test. (d) Expression of the BnaA05.BASS2 in OE lines by Western blot using Flag antibody. The size of the target protein with Flag tag is 45 KD and the red arrow indicates the target protein band. Protein was extracted from 24 DAF seeds. (e) Acquisition of mutant lines based on CRISPR/Cas9 technology. CRISPR targets the eighth exon of BnaA05.BASS2 and BnaC04.BASS2‐1. CR253 and CR313 are homozygous double mutants of BnaA05.BASS2 and BnaC04.BASS2‐1.