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. 2022 Sep 21;20(12):2406–2417. doi: 10.1111/pbi.13922

Figure 4.

Figure 4

BnaBASS2 contributes to oil accumulation during seed development. (a, b) Oil content and weight per seed of developing seeds. Oil content and oil per seed were measured by the GC‐FID method. Seeds were obtained at 21, 27, 31, 36, 41, and 55 DAF. Values are mean ± SD (n = 4–6). ** indicates P < 0.01, based on a student t‐test. (c) and (d), Content of polar lipids and TAG species content in 31 DAF seeds. Values are means ± SD (n = 6–8). Different letters represent significant differences at P < 0.05, based on an ANOVA analysis with the Fisher LSD test. (e) The expression level of genes involved in seed oil synthesis. CALO, caleosin; CPT, CDP choline:diacylglycerol cholinephosphotransferase; DGAT1, diacylglycerol acyltransferase 1; DGAT2, diacylglycerol acyltransferase 2; ENR, enoyl‐ACP reductase; FATA, acyl‐ACP thioesterase A; GPAT9, glycerol‐3‐phosphate acyltransferase 9; GPDH, glycerol‐3‐phosphate dehydrogenase; LPAAT1, lysophosphatidic acid acyltransferase 1; LPAAT2, lysophosphatidic acid acyltransferase 2; LPCAT, lysophosphatidylcholine acyltransferase; MCMT, malonyltransferase; OBO1, oil body oleosin 1; OBO2, oil body oleosin 2; OBO3, oil body oleosin 3; WRI1, wrinkled1. Total RNA was extracted from 31 DAF seeds. Relative expression level was calculated by comparison to actin's expression level. Values are means ± SD (n = 3). Different letters represent significant differences at P < 0.05, based on an ANOVA analysis with the Fisher LSD test.